model p97 stereomicroscope discovery v8 zeiss inverted microscope axioobserver d1 zeiss micromanipulators eppendorf nk2 thin wall capillary tubes  (Carl Zeiss)

Journal: STAR Protocols

Article Title: Protocol for large DNA transgenesis in mice using the Cas9+Bxb1 toolbox

doi: 10.1016/j.xpro.2024.103022

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Article Snippet: Inverted microscope AxioObserver.D1 , Zeiss , .

Techniques: Recombinant, Sequencing, Staining, Embryo Culture, Sterility, Mutagenesis, Plasmid Preparation, DNA Extraction, Software, Imaging, Nucleic Acid Electrophoresis, Electrophoresis, Electroporation, Inverted Microscopy, Transferring

Journal: Scientific Reports

Article Title: Overdriven laser diode optoacoustic microscopy

doi: 10.1038/s41598-023-46855-w

Figure Lengend Snippet: Schematic depiction of the CWLD system for high-speed ALEX and FWM optoacoustic microscopy. ( a ) The system equips three CWLDs, each of which was controlled by a custom-designed electrical circuit driver and received ultrashort trigger signals provided by arbitrary function generators. AL, achromatic doublet lens; AMP, low noise amplifier; AWG, arbitrary waveform function generator; CWLD, continuous wave laser diode; DAQ, data acquisition card; GM, galvanometric mirror scanner; GMC, GM controller; IM, inverted microscope; L, plano-convex lens; M, dielectric mirror; OL, microscope objective lens; PH, pinhole; S, high-precision motorized stage; UT, ultrasound transducer. ( b ) Principle of using the CWLD for alternating laser excitation (ALEX) or Frequency-Wavelength Multiplexed (FWM) optoacoustic microscopy. (Driver inset adapted from ).

Article Snippet: The merging of the beams was achieved by longpass dichroic mirrors (DMLP490R and DMLP550R, Thorlabs) before encountering a set of high-precision galvanometric (galvo) mirrors (6215H Galvanometer Scanners, Cambridge Technologies) in combination with an inverted microscope stand (AxioObserver.D1, Zeiss) for laser-scanning.

Techniques: Microscopy, Inverted Microscopy